Select DNA Replication And Repair Genes Are Transcriptionally Modified By Acute Exercise In Hypoxia

DNA replication and repair in eukaryote cells are critical processes that ensures the correct transfer of material through mitosis. Errors during following cell division may lead to genome instability. PURPOSE: To investigate regulation select gene transcripts aligned exercise hypoxia. METHODS: Twenty-four (n = 24) apparently healthy male participants were recruited (age 28 ± 5 years; mass 74 8 kg; stature 177 6 cm; VO2max hypoxia 45 2 ml·kg-1·min-1: normoxia 60 9 ml·kg-1·min-1), completed 1 hr at a workload corresponding 75% pre-determined (Fio2 0.16%), repeated 0.21%). RNA was extracted from whole blood. Two-colour oligonucleotide microarrays [for primase subunit - PRIM1; Chromatin assembly factor B CHAF1B; Werner syndrome RecQ like helicase WRN] processed on an Agilent microarray scanner, while mRNA analysis conducted using GeneSpring software incorporating checks for data normalisation, quality control, filtering grouping (by experimental condition) statistical (paired t-test with Benjamini & Hochberg False Discovery Rate corrections multiple comparisons). RESULTS: Exercise performed per se downregulated PRIM1 CHAF1B (p 0.03 p 0.01 respectively vs. pre-exercise), no change observed > 0.05). Compared normoxia, WRN differentially 0.05 pre-exercise). CONCLUSION: Acute moderate interferes transcription blood repair. Further work is necessary ascertain consequence these modifications stability.